ELYRA product range combines two powerful technologies dedicated to superresolution microscopy. See beyond conventional light microscopy. Superresolution microscopy enables fluorescence imaging of structures too small for traditional methods, such as deconvolution and confocal microscopy. Carl Zeiss has integrated two methods of superresolution into one turn-key platform. Configure your ELYRA system for superresolution structured illumination (SR-SIM), photoactivated localization microscopy (PALM), or both technologies simultaneously. You can also combine ELYRA system with our laser scanning microscopes LSM 780 or LSM 710 for the benchmark in confocal imaging.
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The tutorial initializes with a pre-activated fluorescent protein specimen appearing in the Specimen window and a dark ribbon structure of the protein Eos positioned on the right-hand side of the tutorial window. In order to operate the tutorial, use the PAL-M Process slider to transition through the various steps in gathering an image. As the slider is moved from one position to another, the action occurring in that particular stage is explained by text boxes in the tutorial and below. In order to choose an alternative specimen, use the Choose a Specimen pull-down menu.
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ELYRA P.1 takes light microscopy to the limit. By localizing small structures and even single molecules, you are able to achieve effective lateral resolutions down to 20 nm.
You are interested in processes that take place near the coverslip. You want to see and measure single molecules in or near the plasma membrane
(lipid rafts, receptor clustering, cell-substrate adhesion sites).
Within this realm, the exclusive PALM technology of ELYRA P.1 takes you into a new world of data quality. Detection with an effective resolution down to
20 nm will show you substructure and patterns where conventional microscopy will reveal “merely” co-localization.
As a single molecule method, PALM is inherently quantitative – every image is a molecular statistics experiment.
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ELYRA S.1 images any fluorophore – with up to twice the resolution of a conventional light microscope.
You have invested a lot of time and energy in producing fusion proteins and multicolor staining protocols that are adapted to your experimental system. Now, with ELYRA S.1, you capture superresolution microscopy data with ease, using samples that may already be available in your lab's freezer. Do you need Z-sectioning for 3D data acquisition? A fast, light-efficient detection? Then ELYRA S.1 is your ideal choice.
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Forget about compromises. ELYRA PS.1 lets you choose the superresolution method that’s best adapted to your specimen – at anytime of an experiment – and with the same superb image quality.
Never have so many imaging modalities been available on a single platform. The complexity of experiments in biomedical research often takes you beyond what a single imaging method can provide. Because you are interested in processes that take place within the context of an entire cell, superresolution imaging will work best if you can image this context at the same time, switching between different imaging methods even in the course of an experiment.
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