Open up the world of multiphoton imaging with ZEISS LSM 710 and LSM 780 microscope systems, used in combination with the NLO add-on.
On top of allround laser scanning microscopy, you see fluorescently labeled structures down to a depth of one millimeter in living tissue.
Detect spectral emission signals of your multi-labeled sample simultaneously with the 32-channel internal detector. Use up to 10 external non-descanned detectors. With this information, you separate signals reliably and produce highly detailed images that are rich in contrast.
And, above all, deeper in insight.
Now, for the first time, you observe neuron networks in brains that are fully intact: the new objective LD Plan-APOCHROMAT 20x/1.0 lets you penetrate into tissue to a depth of up to almost six millimeters and image your sample with excellent sub-cellular resolution.
Experience a New Depth
LD Plan-APOCHROMAT 20x/1.0 opens up a new dimension of optical penetration depth into cleared biological samples – particularly into the brains of mammals. Look deeper into the tissue than ever before - up to six times deeper than with a conventional multiphoton microscope and up to 60 times deeper than with conventional laser scanning microscopy.
You will be impressed by the quality of structural information you retrieve, even from the deepest layers: expect a big push forward, especially in basic neurobiological research and mapping of neuron networks.
Reconstruct in 3D – Fast and Reliable
LD Plan-APOCHROMAT 20x/1.0 is optimized for the scale method.
Use this objective for both laser scanning and multiphoton microscopy and you will appreciate how little hardware you need to set up for multi-fluorescence microscopy, for example - you can even conduct spectral imaging.
Histological cutting procedures are a thing of the past with LD Plan-APOCHROMAT 20x/1.0. Reconstruct tissue elements such as cell groups and cellular components more clearly, faster and more reliably in 3D. There’s no need to apply mechanical cuts so you will avoid gaps that could impede or even prevent reconstruction.
LD Plan-APOCHROMAT 20x/1.0 is the convenient and cost-efficient upgrade for your Axio Examiner system. It only takes the newly designed lens holder to make use of it on your existing system.
Now you can use your Carl Zeiss multiphoton microscope with two NLO lasers at the same time - or use an optical parametric oscillator (OPO) with an NLO laser. It’s your choice: both combinations are fully integrated into your LSM 710 NLO, LSM 780 NLO or LSM 7 MP.
This simultaneous availability of two different excitation wavelengths for multiphoton imaging opens up valuable options for you:
Your system uses different laser wavelengths to excite several fluorescent dyes and proteins simultaneously. The automatic free beam adjustment synchronizes both excitation beams precisely with each other. Manipulate your sample with one wavelength and record the result with another wavelength. Your tests can be reproduced at any time.
The systems are especially well suited for intravital examinations - for example, for research into functional relationships in mouse brains.
The optical parametric oscillator extends the excitation range of your multiphoton microscope to up to 1100nm. Now your system will cover the absorption maximum of red fluorescent proteins such as mCherry, mPlum and tdTomato. This means you can set up long-wavelength excitation precisely and treat your samples with maximum care.
The OPO laser can work with very high intensity. The excitation light doubles and triples its oscillation frequency within the tissue. The non-linear effect of frequency doubling occurs, for example, in muscle fiber and collagen molecules.