LSM 780

Your laser scanning microscope with highest sensitivity

LSM 780

Obtain quantitative information about your cells and individual molecules

The sensitivity of LSM 780 is quite simply outstanding. The GaAsP detector achieves 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.

The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive, the system even allows photon counting.

Awarded quality and sensitivity for your imaging

LSM 780 allows you to achieve images of unparalleled detail, even from the most challenging specimens. The superior quality and sensitivity has been recognized by a distinguished R&D 100 Award in 2011.


Ask your contact at Carl Zeiss about LSM 780.

  • 32-channel GaAsP detector with a typical quantum efficiency of 45% – a significant improvement on the 25% typically achieved by classic PMT detectors
  • Potential for photon counting on GaAsP and the new side PMT
  • With lasers from 355 nm true UV to 660 nm far red, any dye will be excited optimally
  • Simple adaptation to changing acquisition strategies via the ZEN software’s Smart Setup
  • Improved ZEN software with modules for e.g. correlative microscopy, deconvolution and time lapse
  • Improved dynamics for better visualization of weak signals
  • Active cooling together with oversampling and photon counting modes deliver improved signal-to-noise ratio

 

The Scan Module - Core of the LSM 780

1 V/tunable PTC laser ports (405/440, cw/ps; InTune)
2 IR PTC laser port (tunable Ti:Sa)
3 Vis PTC laser ports & Vis AOTF
4 Monitoring diodes
5 InVis TwinGate beamsplitter (upgradable)
6 Vis TwinGate beamsplitter (user exchangeable)
7 Scan mirrors (FOV 20, 6k × 6k)
8 Master pinhole



9   Splitter for external channels
10 Spectral separation and recycling loop
11 Spectral beam guides
12 QUASAR PMT spectral channel # 1
13 QUASAR GaAsP spectral channels # 2–33
14 QUASAR PMT spectral channel # 34
15 Ext. channels (APDs, BiG, FLIM, FCS etc.)

Ask your contact at Carl Zeiss today about LSM 780.

Record 34 channels simultaneously with parallel spectral detection

The maximum possible acquisition speed available to you is determined by the sensitivity of your detection system. When you choose LSM 780, you benefit from new GaAsP detectors that bring a significant improvement on signal-to-noise ratio, allowing you to acquire your image sequences at least twice as fast.

Parallel spectral detection also reads 34 channels simultaneously in Lambda mode: you acquire and separate up to ten colors simultaneously.

 

Ask your contact at Carl Zeiss today about LSM 780.

Simplify your acquisition strategy with Smart Setup and LSM 780

Simply select the color and acquisition strategy for your sample and the Smart Setup tool will define all hardware settings for your LSM 780. Always achieve excellent results: no matter if you acquire molecular processes that are optimized for speed, or high-quality and crosstalk-free acquisition of long-term experiments in developmental biology. And always with the least possible stress from fluorescent light.

ZEN reads the spectral characteristics of several hundreds of dyes from a database. These are the basis for different imaging settings, and the database is updated with new dyes as they become available.

With Smart Setup, users quickly become expert at using LSM 780 because it helps them get to know the characteristics of their samples and leads them through the steps they need to work with the system.

 

Ask your contact at Carl Zeiss today about LSM 780.