The sensitivity of LSM 780 is quite simply outstanding. The GaAsP detector achieves 45 percent quantum efficiency compared to 25 percent typically by conventional PMT detectors. This results in accurate details and contrast-rich images of the challenging specimens you encounter in your live cell imaging.
The system’s illumination and detection design allows you to simultaneously acquire up to ten dyes. You excite any common fluorophore with up to eight different lasers, detecting the signals with the 32 channel GaAsP detector. LSM 780 is so sensitive, the system even allows photon counting.
LSM 780 allows you to achieve images of unparalleled detail, even from the most challenging specimens. The superior quality and sensitivity has been recognized by a distinguished R&D 100 Award in 2011.
Ask your contact at Carl Zeiss about LSM 780.
The Scan Module - Core of the LSM 780
1 V/tunable PTC laser ports (405/440, cw/ps; InTune)
2 IR PTC laser port (tunable Ti:Sa)
3 Vis PTC laser ports & Vis AOTF
4 Monitoring diodes
5 InVis TwinGate beamsplitter (upgradable)
6 Vis TwinGate beamsplitter (user exchangeable)
7 Scan mirrors (FOV 20, 6k × 6k)
8 Master pinhole
9 Splitter for external channels
10 Spectral separation and recycling loop
11 Spectral beam guides
12 QUASAR PMT spectral channel # 1
13 QUASAR GaAsP spectral channels # 2–33
14 QUASAR PMT spectral channel # 34
15 Ext. channels (APDs, BiG, FLIM, FCS etc.)
The maximum possible acquisition speed available to you is determined by the sensitivity of your detection system. When you choose LSM 780, you benefit from new GaAsP detectors that bring a significant improvement on signal-to-noise ratio, allowing you to acquire your image sequences at least twice as fast.
Parallel spectral detection also reads 34 channels simultaneously in Lambda mode: you acquire and separate up to ten colors simultaneously.
Simply select the color and acquisition strategy for your sample and the Smart Setup tool will define all hardware settings for your LSM 780. Always achieve excellent results: no matter if you acquire molecular processes that are optimized for speed, or high-quality and crosstalk-free acquisition of long-term experiments in developmental biology. And always with the least possible stress from fluorescent light.
ZEN reads the spectral characteristics of several hundreds of dyes from a database. These are the basis for different imaging settings, and the database is updated with new dyes as they become available.
With Smart Setup, users quickly become expert at using LSM 780 because it helps them get to know the characteristics of their samples and leads them through the steps they need to work with the system.